Calreticulin expression is associated with gamma-glutamyl transferase and intercellular cell adhesion molecule-1 in biliary atresia
نویسندگان
چکیده
Calreticulin plays a critical role in the etiology of multiple autoimmune diseases. It is unknown, however, whether calreticulin levels are of clinical significance for patients with biliary atresia. This study was to confirm possible correlations between serum calreticulin levels and the immunohistochemical features of biliary atresia. Serum was collected from 44 biliary atresia patients, 15 infants with anicteric choledochal cysts, and 12 healthy controls. Serum calreticulin and liver function tests were detected. Western blot and immunohistochemistry were used to examine and localize calreticulin and intercellular cell adhesion molecule-1 expression in the biliary atresia liver tissues. Calreticulin in serum from biliary atresia patients were elevated. Moreover, the levels of calreticulin in biliary atresia patients correlated with gamma-glutamyl transferase. Calreticulin expression levels were elevated in biliary atresia liver specimens as compared to choledochal cysts infants. Protein expression levels of calreticulin and intercellular cell adhesion molecule-1 were positively correlated. Immunohistochemical detection showed augmented positive immunostaining for calreticulin and intercellular cell adhesion molecule-1 in biliary atresia as compared to choledochal cysts liver tissues. These results suggested that calreticulin may play a potential role in the etiology of biliary atresia. Furthermore, the calreticulin and intercellular cell adhesion molecule-1 as biomarkers may be used to monitor the severity of biliary atresia.
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